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In vitro Clonal Propagation and Organogenesis in Spilanthes acmella (L) Murray: A Herbal Pesticidal Plant of North-East India

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Abstract

Multiple shoots were regenerated in MS medium using different concentrations of BAP and Kn and different combinations of BAP with IAA, NAA and IBA. Highest multiplication of shoots was obtained with BAP (0.75 mg l−1) with 28.4 shoots per explant after 60 days of culture. Shoots rooted best on IBA (0.5 mg l−1), numbering 48.8 per explant. Organogenesis was maximum in callus cultured on MS medium supplemented with BAP (2.0 mg l−1) and IAA (1.0 mg l−1).

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Abbreviations

MS:

Murashige and Skoog

BAP:

6-benzylaminopurine

Kn:

Kinetin

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

NAA:

α-Naphthalene acetic acid

2, 4-D:

2, 4-dichlorophenoxyacetic acid

References

  1. Ansari AH, Mukharya DK & Saxena VK, Indian J Pharm Sci, 50 (1988) 106.

    CAS  Google Scholar 

  2. Ramsewak RS, Erickson AJ & Nair MG, Phytochemistry, 51 (1999) 729.

    Article  PubMed  CAS  Google Scholar 

  3. Pitasawat B, Choochote W, Kanjanapothi D, Panthong A, Jitpakdi A, & Chaithong U, Southeast Asian J Trop Med Public health, 29 (1998) 660.

    PubMed  CAS  Google Scholar 

  4. Murashige T & Skoog F, Physiol Plant, 15 (1962) 473.

    Article  CAS  Google Scholar 

  5. George EF, Plant propagation by tissue culture, Part 1, Exegetics Ltd, Edington (1993).

    Google Scholar 

  6. Handique PJ & Bora P, Curr Sci, 76 (1999) 1245.

    Google Scholar 

  7. Rao LPV & De D N, Plant Sci, 51 (1987) 263.

    Article  CAS  Google Scholar 

  8. Wildi E, Scharrner W & Berger Biiter K, Plant Cell Rep, 18 (1998) 336.

    Article  CAS  Google Scholar 

  9. Luis PBC, Adriane CM, Silvica BRCC & Ana Christina MB, Plant Cell Tiss Org Cult, 56 (1999) 17.

    Article  Google Scholar 

  10. Prakash E, Khan PS, Sairam Reddy P & Rao KR, Plant Cell Rep, 18 (1999) 873.

    Article  CAS  Google Scholar 

  11. Yadav U, Lal M & Jaiswal VS, Sci Hortic, 44 (1990) 61.

    Article  Google Scholar 

  12. Reddy PS, Ramgopal G & Lakshmi Sita G, Curr Sci, 75 (1998) 843.

    Google Scholar 

Download references

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Correspondence to M. C. Kalita.

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Deka, P., Kalita, M.C. In vitro Clonal Propagation and Organogenesis in Spilanthes acmella (L) Murray: A Herbal Pesticidal Plant of North-East India. J. Plant Biochem. Biotechnol. 14, 69–71 (2005). https://doi.org/10.1007/BF03263230

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  • DOI: https://doi.org/10.1007/BF03263230

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