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In vitro Clonal Propagation and Organogenesis in Spilanthes acmella (L) Murray: A Herbal Pesticidal Plant of North-East India

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Abstract

Multiple shoots were regenerated in MS medium using different concentrations of BAP and Kn and different combinations of BAP with IAA, NAA and IBA. Highest multiplication of shoots was obtained with BAP (0.75 mg l−1) with 28.4 shoots per explant after 60 days of culture. Shoots rooted best on IBA (0.5 mg l−1), numbering 48.8 per explant. Organogenesis was maximum in callus cultured on MS medium supplemented with BAP (2.0 mg l−1) and IAA (1.0 mg l−1).

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Abbreviations

MS:

Murashige and Skoog

BAP:

6-benzylaminopurine

Kn:

Kinetin

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

NAA:

α-Naphthalene acetic acid

2, 4-D:

2, 4-dichlorophenoxyacetic acid

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Authors and Affiliations

  1. Department of Biotechnology, Gauhati University, Guwahati, 781 014, Assam, India

    Purabi Deka & M. C. Kalita

Authors
  1. Purabi Deka
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  2. M. C. Kalita
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Correspondence to M. C. Kalita.

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Deka, P., Kalita, M.C. In vitro Clonal Propagation and Organogenesis in Spilanthes acmella (L) Murray: A Herbal Pesticidal Plant of North-East India. J. Plant Biochem. Biotechnol. 14, 69–71 (2005). https://doi.org/10.1007/BF03263230

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  • DOI: https://doi.org/10.1007/BF03263230

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